https://pubmed.ncbi.nlm.nih.gov/30395996/

Chlorine dioxide inhibits the replication of porcine reproductive and respiratory syndrome virus by blocking viral attachment

Zhenbang Zhu 1, Yang Guo 1, Piao Yu 1, Xiaoying Wang 1, Xiaoxiao Zhang 1, Wenjuan Dong 1, Xiaohong Liu 2, Chunhe Guo 3

Affiliations 

• PMID: 30395996
 
• DOI: 10.1016/j.meegid.2018.11.002

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes a great economic loss to the swine industry globally. Current prevention and treatment measures are not effective to control the outbreak and spread of porcine reproductive and respiratory syndrome (PRRS). In other words, new antiviral strategies are urgently needed. Chlorine dioxide (ClO2) is regarded as a broad-spectrum disinfectant with strong inhibitory effects on microbes and parasites. The purpose of this study was to evaluate the inhibitory effects and underlying molecular mechanisms of ClO2 against PRRSV infection in vitro. Here, we identified ClO2 (the purity is 99%) could inhibit the infection and replication of PRRSV in both Marc-145 cells and porcine alveolar macrophages (PAMs). ClO2 could block PRRSV binding to cells rather than internalization and release, suggesting that ClO2 blocks the first stage of the virus life cycle. We also demonstrated that the inhibition exerted by ClO2 was attributed to the degradation of PRRSV genome and proteins. Moreover, we confirmed that ClO2 could decrease the expression of inflammatory cytokines induced by PRRSV. In summary, ClO2 is an efficient agent and potently suppressed PRRSV infection in vitro.

Keywords: Antiviral activity; Chlorine dioxide; PRRSV.

Copyright © 2018 Elsevier B.V. All rights reserved.

Similar articles

• Multiple antiviral approaches of (-)-epigallocatechin-3-gallate (EGCG) against porcine reproductive and respiratory syndrome virus infection in vitro.

  Ge M, Xiao Y, Chen H, Luo F, Du G, Zeng F.Antiviral Res. 2018 Oct;158:52-62. doi: 10.1016/j.antiviral.2018.07.012. Epub 2018 Jul 23.PMID: 30048655
• Inhibitory activity and molecular mechanism of protegrin-1 against porcine reproductive and respiratory syndrome virus in vitro.

  Guo C, Cong P, He Z, Mo D, Zhang W, Chen Y, Liu X.Antivir Ther. 2015;20(6):573-82. doi: 10.3851/IMP2918. Epub 2014 Nov 20.PMID: 25409681
• Inhibition of porcine reproductive and respiratory syndrome virus by Cecropin D in vitro.

  Liu X, Guo C, Huang Y, Zhang X, Chen Y.Infect Genet Evol. 2015 Aug;34:7-16. doi: 10.1016/j.meegid.2015.06.021. Epub 2015 Jun 20.PMID: 26102162
• PRRSV receptors and their roles in virus infection.

  Shi C, Liu Y, Ding Y, Zhang Y, Zhang J.Arch Microbiol. 2015 May;197(4):503-12. doi: 10.1007/s00203-015-1088-1. Epub 2015 Feb 11.PMID: 25666932 Review.
• Overview: Replication of porcine reproductive and respiratory syndrome virus.

  Yun SI, Lee YM.J Microbiol. 2013 Dec;51(6):711-23. doi: 10.1007/s12275-013-3431-z. Epub 2013 Dec 19.PMID: 24385346 Free PMC article. Review.

See all similar articles

Cited by 6 articles

• Inhibitory effect and mechanism of gelatin stabilized ferrous sulfide nanoparticles on porcine reproductive and respiratory syndrome virus.

  Tong T, Deng S, Zhang X, Fang L, Liang J, Xiao S.J Nanobiotechnology. 2022 Feb 5;20(1):70. doi: 10.1186/s12951-022-01281-4.PMID: 35123507 Free PMC article.
• Fresh Produce Safety and Quality: Chlorine Dioxide's Role.

  Malka SK, Park MH.Front Plant Sci. 2022 Jan 11;12:775629. doi: 10.3389/fpls.2021.775629. eCollection 2021.PMID: 35087550 Free PMC article. Review.
• Enhanced Visible-Light Absorption of Fe2O3 Covered by Activated Carbon for Multifunctional Purposes: Tuning the Structural, Electronic, Optical, and Magnetic Properties.

  Tahir D, Ilyas S, Rahmat R, Heryanto H, Fahri AN, Rahmi MH, Abdullah B, Hong CC, Kang HJ.ACS Omega. 2021 Oct 18;6(42):28334-28346. doi: 10.1021/acsomega.1c04526. eCollection 2021 Oct 26.PMID: 34723030 Free PMC article.
• Antiviral Effectivity of Favipiravir Against Peste Des Petits Ruminants Virus Is Mediated by the JAK/STAT and PI3K/AKT Pathways.

  Zhang W, Deng H, Liu Y, Chen S, Liu Y, Zhao Y.Front Vet Sci. 2021 Sep 6;8:722840. doi: 10.3389/fvets.2021.722840. eCollection 2021.PMID: 34552976 Free PMC article.
• Kinetics and Mechanisms of Virus Inactivation by Chlorine Dioxide in Water Treatment: A Review.

  Ge Y, Zhang X, Shu L, Yang X.Bull Environ Contam Toxicol. 2021 Apr;106(4):560-567. doi: 10.1007/s00128-021-03137-3. Epub 2021 Feb 25.PMID: 33629148 Free PMC article. Review.